Part:BBa_K3225015:Design

J23101-LuxR-pLux-LacZ

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We built this device with Gibson assembly, therefore there is no biobrick scar between two parts.

Source

Sequence of LuxR and pLux is from part BBa_C0062 and BBa_R0062.And LacZ is from BBa_I732017. The physical samples of them is de-novo synthesis.